defter*
defter / katalog / MBG 230
MBG 230

Experimental Approaches in Molecular and Cellular Biology

MBG 230 is the course where you stop treating lab techniques as black boxes and start understanding *why* each one works — the physical-chemical logic behind restriction enzymes, cloning, PCR, sequencing, and the cell-based assays that show up everywhere in modern molecular biology. You'll work through Brown's gene cloning textbook alongside primary literature, give presentations, and do homework and a project aimed at interpreting real experimental data rather than memorizing protocols. It's the methods backbone that makes upper-year MBG labs and research electives actually make sense, and it's where you learn to read a figure skeptically instead of trusting whatever the authors claim.

Credit3ECTS5FacultyFaculty of ScienceBölümMolecular Biology and GeneticsPreMBG 101

Önerilen kaynaklar 2 kitap

📕
Zorunlu
Gene Cloning and DNA Analysis: An Introduction
T. A. Brown
2016 · 7th edition
📖
Önerilen
Principles of Gene Manipulations and Genomics
Eds: S.B. Primrose, R.M. Twyman
2006 · Blackwell Publ

Haftalık müfredat 14 hafta

Hafta 1
• Why Gene Cloning and DNA Analysis are Important • Laboratory tools for molecular genetic applications (Restriction enzymes, vectors)
Hafta 2
• Vectors for Gene Cloning: Plasmids and Bacteriophages
Hafta 3
• Purification of DNA from Living Cells • Manipulation of Purified DNA
Hafta 4
• Introduction of DNA into Living Cells
Hafta 5
• Cloning Vectors for Escherichia coli • Cloning Vectors for Eukaryotes
Hafta 6
• How to Obtain a Clone of a Specific Gene (Libraries)
Hafta 7
• The Polymerase Chain Reaction • Sequencing Genes and Genomes
Hafta 8
• Production of Protein from Cloned Genes
Hafta 9
• Gene Cloning and DNA Analysis in Medicine
Hafta 10
• Gene Cloning and DNA Analysis in Forensic Science
Hafta 11
• Cell growth and cytotoxicity assays
Hafta 12
• Cell adhesion, invasion, motility, apoptosis assays
Hafta 13
• Nucleic acids as therapeutic agents
Hafta 14
• Transgenic animals

Ders notları — henüz yok

MBG 230 için defter ekibi henüz not yazmadı.

İlk dosyayı sen atarsan — not, slayt, geçmiş sınav, çözüm, cheat-sheet, ne varsa — defter ekibi öğrenci paylaşımlarından bu dersin notlarını yazar. Drive linki / PDF / ZIP, hepsi olur.

← katalog

⚠️ FZ engelleyen şartlar

You have to get at least 55/100 from weighted average of two midterm exams plus homework assignments and presentations.

Hocalar 0 bu dönem · 1 geçmiş

Geçmişte ders veren (1 kişi)
Işık Yuluğ