Special Techniques in Molecular Genetics

Principles of specific methods used in the molecular genetics, laboratory tools for molecular genetic applications, restriction enzymes, cloning vectors, gene Analysis methods, DNA cloning, gene libraries and library screening, PCR and other applications, expression of cloned genes in cultured cells, isolation and characterization of gene transcripts, RNA analysis, microarray analysis, proteomics , RNA world and RNA knockdown strategies, gene manipulations in animals, gene and cellular therapies. Discussions of selected research papers. (No lab session.)